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KMID : 0545119940040030233
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 3 p.233 ~ p.234
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An Improved and Efficient Method for Cosmid Cloning
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LEE BAEK-RAK
AERNARD WEISBLUM
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Abstract
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A general improved procedure for preparation of a cosmid library based on the use of a pBLcosT vector was described. The vector was modified to contain 2 tandem Xcml sites and was digested with Xcml to yield 2 terminal 3´T overhangs, capable of ligation with the insert that contains 2 terminal complementary 3´A overhangs. The resultant ligation mixture was packaged and a cosmid library in Escherichia coli was established.
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